TECHNICAL CHARACTERISTICS chemical properties | purity | stability | formula | safety

Hygromycin is suitable for research purposes only. It must not be used on humans.

Hygromycin B is produced from proprietary Streptomyces mutant strains in our own manufacturing facilities.

HygroGold is the commercial name for highly purified hygromycin B solution sold by InvivoGen (100mg/ml active compound - pale yellow solution).


Chemical name: O-6-Amino-6-deoxy-L-glycero- D-galacto- heptopyranosylidene- (1-2-3)-O-beta-D-talopyranosyl (1-5) -2-deoxy-N3-methyl-D-streptamine


Formula : C20H37N3O13, HCl

Molecular weight: 527.53 ( 563.5 with HCl )

CAS: 31282-04-9

Solubility: soluble in water

pKa values: 7.1 and 8.8 in water


Hygromycin purity is routinely measured by HPLC in our production facility, with some advanced controls using NMR. High purity (>98%) guarantees consistent results.

Hygromycin purity varies from from one supplier to another. The color of hygromycin solutions is a good purity indicator: the clearer it is, the purer. Therefore, "caramel colored" solutions are of inferior grade. The color difference may not be very dramatic with a freshly made low-concentration hygromycin solution, but with some aging the difference is striking.

* Impurities present in the hygromycin preparation are easily visualized because their oxydation over time darken the solution. To speed up this process for a quick visual test, the solution can be artificially aged by heating-up the solution for a few minutes at 60°C.


Hygromycin is stable for at least one year at –20°C, three months at room temperature, and at least one month at 37°C. Hygromycin B is sensitive to high concentrations of acid, but short-term exposure to dilute acids can be tolerated.



CAUTION: The hygromycin B powder is highly allergenic. Avoid contact or inhalation. Wear respirator, paper jacket and pants, headcovering and lab gloves when handling this material. Use local exhaust ventilation.

Harmful if swallowed, inhaled or absorbed through skin.

Hygromycin powder often causes irritation to eyes, skin, mucus membranes and upper respiratory tract if it is not handled with great care by qualified personnel.

See also Material Safety DataSheet.