MECHANISM OF ACTION
Hygromycin B is an aminocyclitol antibiotic with broad spectrum activity against prokaryotes and eukaryotes (1,2). Hygromycin B strongly inhibits protein synthesis through a dual effect on mRNA translation (3,4). Like other aminoglycoside antibiotics, hygromycin B induces misreading of aminoacyl-tRNA by distorting the ribosomal A site (decoding center) (3,5,6,7). Hygromycin B also affects the ribosomal translocation process (3,8,9). In the presence of the antibiotic, mRNA is often mistranslocated, being moved more or less than the three necessary bases.
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